Human Taste Cells Regenerate in a Dish

This advance ultimately will assist efforts to prevent and treat taste loss or impairment due to infection, radiation, chemotherapy and chemical exposures.

"People who undergo chemotherapy or radiation therapy for oral cancer often lose their sense of taste, leading to decreased interest in food, weight loss, and malnutrition," said lead author Doctor M. Hakan Ozdener, a cellular biologist at Monell. "The success of this technique should provide hope for these people, as it finally provides us with a way to test drugs to promote recovery."

Taste cells are found in papillae, the little bumps on our tongues. These cells contain the receptors that interact with chemicals in foods to allow us to sense sweet, salty, sour, bitter, and umami. They also are among the few cells in the body with the special capacity to regenerate, with new taste cells maturing from progenitor 'stem' cells every 10-14 days.

For many years, it was believed that taste cells needed to be attached to nerves in order to both function properly and regenerate. For this reason, scientists thought that it was not possible to isolate and grow these cells in culture, which limited the scope of studies to understand how human taste cells function. To dispel the long-held belief, the scientists first demonstrated in 2006 that taste cells from rats could successfully be maintained in culture. In the current study, they then applied that methodology to a more clinically relevant population – humans.

Taking tiny samples of tongue tissue from human volunteers, the researchers first adapted existing techniques to demonstrate that the human taste cells indeed can regenerate in culture.

They went on to show that the new taste cells were functional, maintaining key molecular and physiological properties characteristic of the parent cells. For example, the new cells also were activated by sweet and bitter taste molecules. "By producing new taste cells outside the body, our results demonstrate that direct stimulation from nerves is not necessary to generate functional taste cells from precursors," said Ozdener.

The establishment of a feasible long-term taste cell culture model opens a range of opportunities to increase understanding of the sense of taste. The cells also can be used to screen and identify molecules that activate the taste receptors; one such example might be a salt replacer or enhancer. "The model will help scientists identify new approaches to design and establish cell culture models for other human cells that previously had resisted viable culture conditions," said Ozdener.; Source: Monell Chemical Senses Center