High Performance Liquid Chromatographs are developed and manufactured by Institute of Chromatography ECONOVA, Ltd., Novosibirsk, Academpark hi-tech company.

These science intensive instruments designed with unique possibility to perform analyses of hundreds of chemicals by one fast run: no reference materials need and the only simple complex validation procedure is applied in accordance with renewed, certified electronic data base.

"MiLiChrom® А-02" - fully portable and automated Micro-column Liquid Chromatograph is intended to use in the stationary and mobile analytical laboratories:

- No reference materials need;

- The same HPLC-UV D-Base for any "MiLiChrom® А-02";

- Hundreds of chemicals by one fast HPLC procedure;

- Simple complex validation procedure;

- Lower analysis cost.

Within the frame of the whole-genome sequencing of helminthiasis, a novel RT-PCR kit is developed for the simultaneous (differential) detection of four parasites, which cause Opisthorchiasis: O. felineus, O. viverini, M. bilisand C. sinensis.

The kit will be launched on the market in next year and it will give the customers the opportunities for fast and qualitative detection of patogenic DNA in human specimen without their additional enrichment and false-positive results.

High Performance Liquid Chromatographs are developed and manufactured by Institute of Chromatography ECONOVA, Ltd., Novosibirsk hi-tech company.

These science intensive instruments designed with unique possibility to perform analyses of hundreds of chemicals by one fast run: no reference materials need and the only simple complex validation procedure is applied in accordance with renewed, certified electronic data base.

"MiLiChrom® А-02" - fully portable and automated Micro-column Liquid Chromatograph is intended to use in the stationary and mobile analytical laboratories:

- No reference materials need

- The same HPLC-UV D-Base for any "MiLiChrom® A-02"

- Hundreds of chemicals by one fast HPLC procedure

- Simple complex validation procedure

- Lower analysis cost

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

Reagents:

2'-Deoxynucleoside-5'-triphosphates:

- commonly used substrates for DNA synthesis;

- essential components for widespread molecular biology applications such as PCR for DNA research and medical diagnostics, DNA hybridisation probes production etc.

dATP - 2'-deoxyadenosine-5'-triphosphate

dCTP - 2'-deoxycytidine-5'-triphosphate

dGTP –2'-deoxyguanosine-5'-triphosphate

TTP - thymidine-5'-triphosphate

dUTP – 2'-deoxyuridine-5'-triphosphate

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Quality of dNTPs:

Purity of dNTPs is 98 – 99 % according to HPLC analysis.

All product are free of DNases, RNases and phosphatases activities.

All product are free of pyroposphate.

All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Modified Deoxynucleotide-5'-Triphosphate from BIOSAN, Ltd.:

AA-dUTP — 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate

AA-UTP — 5-(3-aminoallyl)-2'-uridine 5'-triphosphate

AZTTP — 3'-Azido-3'-deoxythymidine-5'-triphosphate

Bio-11-dUTP — 5-[N-(N-biotinyl-Є-aminocaproyl)-3-aminoallyl]-2'-deoxyuridine-5'-triphosphate

Br-dUTP — 5-Bromo-2'-deoxyuridine-5'-triphosphate

7-deaza-dGTP — 7-deaza-2'-deoxyguanosine-5'-triphosphate

FITC-4-dUTP — Fluorescein-6-aminothiocarbonyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]

Flu-12-dUTP — Fluorescein-5(6)-carboxamidocaproyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]

Rho-5-dUTP — Tetramethylrhodamin-5(6)-[5-(3-carboxaminoallyl)-2'-deoxyuridine 5'-triphosphate

8-oxo-dGTP — 8-oxo-2'-deoxyguanosine-5'-triphosphate

ddNTPs — 2',3'-dideoxynucleoside-5'-triphosphates (ddATP, ddGTP, ddCTP and ddUTP)

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Purity of dNTPs is 98 — 99 % according to HPLC analysis.

All product are free of DNases, RNases and phosphatases activities.

All product are free of pyroposphate.

All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Custom Oligonucleotide Synthesis

Custom oligonucleotide synthesis in wide range of length, scale and different chemical modifications.

Enzymes from BIOSAN, Ltd.:

— DNA polymerase of the archaeon Pyrococcus furiosus

— M-MLV Reverse Transcriptase (RNase H Minus)

— T4 Polynucleotide Kinase

— T4 DNA Ligase

— T7 RNA Polymerase

— Taq DNA Polymerase

— Taqm DNA polymerase

— Thermostable DNA ligase of the archaeon Pyrococcus abyssi

DNA Ladders from BIOSAN, Ltd.:

- 100 bp DNA Ladder (400х2)

- 100 bp DNA Ladder (500х2)

- pBlueSK DNA/MspI Marker

- Lamda DNA/EcoRI+HindIII Marker

- 100+50 bp DNA Ladder

- DNA Ladder BX100-7

- 50 bp DNA Ladder

- 250 bp DNA Ladder

- 1 kb DNA Ladder

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

2'-Deoxynucleoside-5'-triphosphates

- commonly used substrates for DNA synthesis;

- essential components for widespread molecular biology applications such as PCR for DNA research and medical diagnostics, DNA hybridisation probes production etc.

dATP - 2'-deoxyadenosine-5'-triphosphate

dCTP - 2'-deoxycytidine-5'-triphosphate

dGTP –2'-deoxyguanosine-5'-triphosphate

TTP - thymidine-5'-triphosphate

dUTP – 2'-deoxyuridine-5'-triphosphate

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Quality of dNTPs:

Purity of dNTPs is 98 – 99 % according to HPLC analysis.

- All product are free of DNases, RNases and phosphatases activities.

- All product are free of pyroposphate.

- All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

- 100 bp DNA Ladder (400х2)

- 100 bp DNA Ladder (500х2)

- pBlueSK DNA/MspI Marker

- Lamda DNA/EcoRI+HindIII Marker

- 100+50 bp DNA Ladder

- DNA Ladder BX100-7

- 50 bp DNA Ladder

- 250 bp DNA Ladder

- 1 kb DNA Ladder

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

— DNA polymerase of the archaeon Pyrococcus furiosus

— M-MLV Reverse Transcriptase (RNase H Minus)

— T4 Polynucleotide Kinase

— T4 DNA Ligase

— T7 RNA Polymerase

— Taq DNA Polymerase

— Taqm DNA polymerase

— Thermostable DNA ligase of the archaeon Pyrococcus abyssi

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

• AA-dUTP — 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate
• AA-UTP — 5-(3-aminoallyl)-2'-uridine 5'-triphosphate
• AZTTP — 3'-Azido-3'-deoxythymidine-5'-triphosphate
• Bio-11-dUTP — 5-[N-(N-biotinyl-Є-aminocaproyl)-3-aminoallyl]-2'-deoxyuridine-5'-triphosphate
• Br-dUTP — 5-Bromo-2'-deoxyuridine-5'-triphosphate
• 7-deaza-dGTP — 7-deaza-2'-deoxyguanosine-5'-triphosphate
• FITC-4-dUTP — Fluorescein-6-aminothiocarbonyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]
• Flu-12-dUTP — Fluorescein-5(6)-carboxamidocaproyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]
• Rho-5-dUTP — Tetramethylrhodamin-5(6)-[5-(3-carboxaminoallyl)-2'-deoxyuridine 5'-triphosphate
• 8-oxo-dGTP — 8-oxo-2'-deoxyguanosine-5'-triphosphate
• ddNTPs — 2',3'-dideoxynucleoside-5'-triphosphates (ddATP, ddGTP, ddCTP and ddUTP)

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Purity of dNTPs is 98 — 99 % according to HPLC analysis.

All product are free of DNases, RNases and phosphatases activities.

All product are free of pyroposphate.

All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Custom Oligonucleotide Synthesis:

Custom oligonucleotide synthesis in wide range of length, scale and different chemical modifications.

MBU has menu of ELISA kits for detection of hepatitis B markers in human sera or plasma.

Features and benefits:

 Kits provide all the necessary components to perform the test: The test-kits are fully completed with colour-coded reagents in order to prevent reagents confusing or mixing up.

 For more convenience – ready-to-use reagents format: The kits can be offered with ready-to-use conjugates and chromogen.

 For economy and workload flexibility – stripped plates, in 96-well and 192-well formats: Use the required number of strips as new specimens are delivered to the laboratory.

 High sensitivity and specificity

 High reproducibility of results

 Easy shipping and storage: The kits can be transported and stored at 17-27°С up to 10 days.

 Reasonable price margin and reliable quality

 Test kits features:

 HBs-antigen-DS-strip confirmatory test kit is an indirect, competitive enzyme-linked immunosorbent assay intended to confirm the presence of HBsAg in human serum or plasma and combines direct and competitive ELISA assay for exclusion of false-positive results. Incubation time: 2 h 20 min (80+40+20). Shelf life: 12 months.

 InVitroLogic HBsAg is a sandwich-type enzyme-linked immunosorbent assay for the detection of HBsAg. Incubation time using shaker: sensitivity 0.05 IU/ml - 45 min (20+10+15) at 42 С or 1 h 5 min (30+20+15) at 37С; sensitivity 0.01 IU/ml - 1 h. 5 min (40+20+15) at 42 С or 1 h 45 min (60+30+15) at 37 С. Shelf life: 12 months.

 InVitroLogic anti-HBs is a sandwich-type enzyme-linked immunosorbent assay for the detection and quantitative determination of total anti-HBs. Incubation time: 1 h 20 min (40+20+20). Shelf life: 12 months.

 InVitroLogic anti-HBcore-IgM is an indirect solid phase enzyme-linked immunosorbent assay for the detection of anti-HBcore IgM. Incubation time: 1 h 15 min (30+30+15). Shelf life: 12 months.

 InVitroLogic anti-HBcore is an indirect solid phase enzyme-linked immunosorbent assay for the detection of total anti-HBcore. Incubation time: 1 h 10 min (60+10). Shelf life: 12 months.

 InVitroLogic anti-HBe is a sandwich-type enzyme-linked immunosorbent assay for the detection of total anti-HBe. Incubation time: 1 h 20 min (40+20+20). Shelf life: 12 months.

 The products have the certification of country manufacturer (Russia) and have not CE-marking.

MBU has menu of ELISA kits for detection of HIV-infection markers: 3rd and 4th-generation HIV ELISA.

Features and benefits:

 Kits provide all the necessary components to perform the test: The test-kits are fully completed with colour-coded reagents in order to prevent reagents confusing or mixing up.

 For more convenience – ready-to-use reagents format: The kits can be offered with ready-to-use conjugates and chromogen.

 For economy and workload flexibility –  stripped plates, in 96-, 192- and 480-well formats: Use the required number of strips as new specimens are delivered to the laboratory.

 High sensitivity and specificity

 High reproducibility of results

 Easy shipping and storage: The kits can be transported and stored at 17-27°С up to 10 days.

 Reasonable price margin and reliable quality

 Test kits features:

 InVitroLogic HIV-1,2-Ab kit is 3rd generation HIV kits, andwich-type enzyme-linked immunosorbent assay. Incubation time: 1 h 15 min (30+30+15). Shelf life: 12 months.

 HIV-1,2-Ag/Ab kit is 4th generation HIV kit, a sandwich-type enzyme-linked immunosorbent assay, 10 or 20 pg/ml. Incubation time: 1 h 25 min (60+10+15). Shelf life: 12 months.

The products have the certification of country manufacturer (Russia) and have not CE-marking.

Menu of reagents for Immunoassays from BIOSAN, Ltd.:

Monoclonal antibodies (MAB) for diagnostics of tick-borne encephalitis virus (TBEV):

- MAB FV-5 (2H3)

Mouse MAB to envelope protein E of TBEV (strain Sofjin), IgM class, have neutralizing properties, complex-specific activity (i.e. react with all strain of TBEV and other viruses of TBEV complex), high association constant (Ka = 1,8 x 109 M-1).

- MAB FVN-22 (14D5)

Mouse MAB to envelope protein E of TBEV (strain Sofjin), IgG1 subclass, have neutralizing properties, partially complex specific activity (i.e. react with all strain of TBEV and other viruses of TBEV complex, except Langat virus ), high association constant (Ka = 1,8 x 109 M-1).

Menu of reagents for Immunoassays from BIOSAN, Ltd.:

- MAB HV-7: Mouse MAB for HBsAg (ay subtype), IgG class, specific for a determinant.

- MAB HV-11: Mouse MAB for HBsAg (ay subtype), IgG class, specific for y determinant.

- MAB HV-42: Mouse MAB for HBsAg (ad subtype), IgG class, specific for y determinant.

Monoclonal antibodies (MAB) for human immunoglobulins from BIOSAN, Ltd.:

MAB G5 are specific for kappa light chain of human immunoglobulins, belong to IgG class. MAB G5 labeled with horseradish peroxidase or other labels have been used as second antibody for diagnostics of various diseases, where the detection of specific antibodies is necessary.

Recombinant-HIV-1,2-DSM

ELISA kit for detection of antibodies to HIV-1 and HIV-2 in human serum or plasma

192 tests

Recombinant-HIV-1,2-DSM (stripped)

ELISA kit for detection of antibodies to HIV-1 and HIV-2 in human serum or plasma

(12x8) 96 tests

HBs/HBc-Ag/Ab ELISA kit for the detection of HBsAg and/or total antibodies to HBcoreAg in human serum or plasma.

An upgraded sandwich-type HBV ELISA kit is designed by the company. The kit serves as a tool for diagnostics of HBV during the early stages of the infection and it allows to reduce diagnostic (seroconversion) window.

Features and benefits:

 HBV fourth-generation ELISA has been developed for simultaneous or differential detection of HBsAg and/or total antibodies to hepatitis B virus core antigen (anti-HBcAg) in human serum or plasma.

 High sensitivity and specificity: sensitivity level of detection of HBsAg – 0.05 IU/ml.

 High reproducibility of results

 Immunosorbent: Plate coated with a mixture of polyclonal antibodies to hepatitis B virus surface antigen HBsAg and recombinant hepatitis B virus core antigen (HBcAg).

 Conjugate 1 - Biotin conjugate: monoclonal antibodies to HBsAg conjugated with biotin, in buffer.

 Conjugate 2 - Streptavidin-HRP conjugate: streptavidin conjugated with horseradish peroxidase, in buffer.

 Conjugate 3 – Ag-HRP conjugate: recombinant hepatitis B virus core antigen (HBcAg) conjugated with horseradish peroxidase, in buffer.

 Incubation time using shaker: 1 h 20 min (40+20+20) at 42 С.

 Shelf life: 12 months.

The product has not CE mark.

Control Panels are panels of human sera intended for use in external control and testing the work standards of clinical diagnostic laboratories. Control Panels are used to demonstrate serological lab proficiency.

Control Panels can also be used to challenge test kits performance, sensitivity, and specificity, to evaluate and validate new test methods, new equipment, show up the mistakes occurred during ELISA test kits performance, correct these mistakes, and train laboratory personnel.

Control Panels are included in the program of The Federal System External Quality Assessment (FSVOK) of Federal Service on Surveillance in Healthcare and Social Development of Russian Federation and local EQA programs. Each Control Panel contains 4 or 8 positive members and 1-4 negative members.

All sera are thoroughly characterized by a range of commercially available test kits. Qualification panels have similar intended use to that of the Control panels.

The sera included into the control panel are differentiated to positive and negative. The sera included into the qualification panel are also differentiated to positive and negative, and additionally positive sera are ranged in the order of marker concentration increasing.

Each Qualification Panel contains 4 positive members, which represent serial dilutions of human serum and 1 negative member.

FOR DAY-TO-DAY QUALITY CONTROL OF ELISA PERFORMANCE INSIDE CLINICAL LABORATORY

External run controls are intended:

q For monitoring test kits performance presision, stability and reproducibility;

q For showing up accidental and systematic errors occurring during testing and determination of errors origin;

q For troubleshooting an assay performance.

Laboratory external control consists of 24 equal members representing the preparations lyophilized from inactivated human serum. Each serum contains certain infectious disease marker, e.g. viral antigen, antibodies to viral antigen.

The liquid external run multicontrols are coming soon.

The products have not CE-marking.

Reference and Performance Panels are panels of human sera intended for use in control serological assays. Each panel consists of a thoroughly characterized series of serum collected from different individuals.

The sera members of panels represent the full reactivity range of the assay, including negative reactivity. Each panel also contains a comprehensive datasheet with testing results from a variety of commercially available test kits.

Reference and Performance Panels are used to challenge test kits performance, sensitivity, and specificity. Panels can be used to evaluate and validate new test methods, new equipment, demonstrate lab proficiency, and train laboratory personnel. As the panels represent the Russian collection of sera containing markers of infectious diseases and containing no such markers, they can be helpful for international users during for the test kits characteristics investigation.

These panels were developed and manufactured under the requirements specification of Tarasevich State Institute of Standardization and Control of Medical Biological Preparations. Each taken separately Reference Panel of sera was been recognized as Immunobiological Standard and has specific branch standard number (OCO).