Harvested infected cells are treated to remove cellular components and ultracentrifuged to remove whole virus particles. Pure hexon antigen is obtained from the resulting supernatant by ion exchange chromatography and the antigen presented in 30mM BIS Tris buffer , pH 6.8
Product category: Equipment and systems for HIV determination units, Reagents for HIV determination
Affinity purified anti-HIV-1-gp120 D7324
This antibody product is produced by first immunising sheep with a single synthetic peptide which has the amino acid sequence : APTKAKRRVVQREKR This amino acid sequence corresponds to amino acid numbers 497-511 in the envelope gene gp120 protein of the BH-10 strain of HIV-1 (see Ratner , L. et al., Nature, 313, 277-284 (1985). The antibodies are isolated from the sheep hyperimmune serum by affinity chromatography using the above specified synthetic peptide coupled to Sepharose.
Founded in 1978, Aalto Bio Reagents Ltd is a leading provider of raw materials to the in-vitro diagnostics industry and to research laboratories globally. We serve the largest multinational companies in our industry with a broad range of purified human proteins; polyclonal antisera; purified monoclonal and polyclonal antibodies; fungal, parasitic, bacterial and viral antigens; and disease state plasma for in-vitro diagnostic application. Aalto Bio Reagents Ltd. is ISO 9001:2008 certified.
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