In 1945, Coombs, Mourant and Race described the use of anti-human globulin serum for detecting red cell-bound non-agglutinating antibodies. In 1957, Dacie et al showed that the antibodies present in antiglobulin sera were directed against certain components of complement. Anti-human globulin reagents detect non-agglutinating antibody molecules as well as molecules of complement attached to red cells following in vivo or in vitro antigen-antibody reactions.
When used by the recommended techniques, the reagents will react with immunoglobulins and/or complement attached to the red cell surface, resulting in agglutination (clumping) of adjacent sensitised cells. Cells not sensitised will not be agglutinated.
Lorne Polyspecific Anti-Human Globulin Elite Clear and Anti-Human Globulin Elite Green reagents contain anti-IgG derived from rabbits with non-specific activity removed by absorption and murine monoclonal IgM anti-C3d, Clone BRIC-8. The antibodies are diluted in a buffered solution containing bovine albumin. Each reagent is supplied at optimal dilution for use with spin tube techniques (DAT & IAT).
The reagents will cause direct agglutination (clumping) of test red cells that carry the corresponding ABO antigen.
No agglutination generally indicates absence of the corresponding ABO antigen. Lorne Monoclonal IgM ABO blood grouping reagents contain murine monoclonal antibodies diluted in a phosphate buffer containing sodium chloride, EDTA and bovine albumin. Each reagent is supplied at optimal dilution for use with slide, tube, gel card & microplate techniques.
A1 antigen is a subgroup of A and was discovered in 1910. Anti-A1 is usually non-reactive at 37ºC, however examples reactive at 37ºC and predominately IgM can cause in vivo red blood cell destruction. About 78% of group A people are A1 and 22% are A2, similar proportions apply among AB people.
The reagent will cause agglutination (clumping) of test red cells, that carry the A1 antigen, after centrifugation. No agglutination generally indicates the absence of the A1 antigen.
Lorne Anti-A1 Lectin blood grouping reagent is prepared from an extract of Dolichos biflorus seeds, diluted with a sodium chloride solution containing bovine albumin. The reagent is supplied at optimal dilution for use with slide & tube techniques.
Levine and Stetson discovered the Rh blood group system in 1940. Apart from D the other major Rh antigens are C, E, c and e. The D antigen is highly immunogenic; the C and e antigens are less immunogenic than E and c. The corresponding antibodies are all clinically significant since they may cause both Transfusion Reactions and Haemolytic Disease of the Newborn.
The reagents will cause direct agglutination (clumping) of test red cells that carry the C and/or D and/or E antigen. No agglutination generally indicates the absence of the corresponding Rh antigen.
Lorne Monoclonal IgM Anti-Rh blood grouping reagents are low protein reagents containing human monoclonal antibodies diluted with sodium chloride, bovine albumin and macromolecular potentiators. Each reagent is supplied at optimal dilution for use with slide, tube, gel card and microplate techniques.
The Lua and Lub antigens were reported in 1945. The expression of the antigens on the red cells can vary widely from person to person. Anti-Lua is not generally associated with Haemolytic Transfusion Reactions but anti-Lub has been. Both anti-Lua and anti-Lub have been implicated in Haemolytic Disease of Newborn.
The reagents will cause indirect agglutination (clumping) of test red cells, that carry the corresponding Lutheran antigen, in the antiglobulin phase of testing. No agglutination generally indicates the absence of the corresponding Lutheran antigen.
Lorne Polyclonal Anti-Lua blood grouping reagent is prepared from human serum diluted in a sodium chloride solution containing macromolecular potentiators and bovine albumin. Each reagent is supplied at optimal dilution for use with indirect Coombs tube test and gel card techniques.
Lorne Laboratories is a specialist manufacturer of blood grouping reagents and diagnostic test kits. We are also distributors of laboratory equipment including refrigerators, freezers and slide strainers. Our products are used by blood transfusion professionals, laboratories and pharmacies in the UK, and in more than 80 countries around the world.
Lorne can produce diagnostic test kits and reagents in vials, or in bulk if required, each carefully quality controlled from the sourcing of the raw materials to final filling under laminar flow.