Manufacturers OEM

PCR Biosystems Ltd.

London Bioscience Innovation Centre,2, Royal College Street , NW1 0NH London
United Kingdom

Telephone +44 20 76913563
Fax +44 20 76812186
info@pcrbio.com

This company is co-exhibitor of
The Gambica Association Limited

Trade fair hall

  • Hall 1 / E05
 Interactive Plan

Hall map

MEDICA 2016 hall map (Hall 1): stand E05

Fairground map

MEDICA 2016 fairground map: Hall 1

Our range of products

Product categories

  • 03  Diagnostics
  • 03.01  Clinical chemistry
  • 03.01.04  Enzymes

Our products

Product category: Enzymes

cDNA Synthesis

When synthesizing a protein, DNA is transcribed into mRNA which is then translated into a protein. One difference between eukaryotic and prokaryotic genes is that eukaryotic genes often contain introns which are not coding sequences, in contrast with exons, which are DNA coding sequences. During transcription, intronic RNA is excised from the RNA primary transcript and the remaining pieces of the RNA primary transcript are spliced back together resulting in processed mRNA. The mRNA code is then translated into an amino acid chain that comprises the newly made protein.

Using reverse transcriptase polymerases, DNA can be synthesised from mRNA and total RNA. Thus it is a 'complementary' copy of the mRNA, and is thus called complementary DNA (cDNA). cDNA forms the substrate for the majority of qPCR gene expression experiments.

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Product category: Enzymes

DNA Markers

Room temperature stable
High intensity bands for easy size determination
Ready to load
Multiple dyes for simple migration observation
PCRBIO Ladders are designed for size determination and DNA quantification using agarose electrophoresis.

All 4 PCRBIO Ladders are manufactured using a combination of plasmid restriction digest fragments and PCR amplification products.

The ladders are resuspended in sample buffer ready for immediate gel loading. The buffer formulation also allows for greater room temperature stability compared to conventional DNA ladders.

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Product category: Enzymes

Endpoint PCR

The process of DNA manipulation has been in use for over half a century, since the advent of restriction enzymes. However it was not until Karry Mullis demonstrated the process of Polymerase Chain Reaction (PCR) in 1983 that the manipulation of DNA was sufficiently functional for it to change the landscape of biological research.

PCR continues to be the gold standard for DNA manipulation and is still present in the majority of biological research laboratories. Although PCR is principally unchanged the technique has developed over time so that many different polymerases are available for use depending on the application. The functionality for these applications also continues to improve as development scientists find ways to improve the reliability, yield, specificity, speed and sensitivity of DNA Polymerases. PCR Biosystems offer a range of polymerases to suit all your needs. These are developed by applying the latest advances in polymerase and buffer system technology to provide DNA polymerases which scientists can rely on.

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Product category: Enzymes

Real-time PCR

Real-time PCR is a fast developing field with wide ranging applications for researchers. From gene expression analysis from cDNA, population genotyping studies, to the multiplex detection of several disease targets real-time PCR has opened the door to a world of possibilities.

Real-time analysis is broadly divided into two areas; probe and intercalating dye based assays. The two techniques have many overlapping applications.

For the majority of applications we recommend the use of an intercalating dye technology rather than costly dual labelled probes. Using an intercalating dye, such as SyGreen, rather than a hydrolysis probe (such as Taqman®) has the added benefit of post amplification melt profile analysis. Intercalating dye real-time PCR also has the advantage of being able to cycle faster than with hydrolysis probes, this is due to the slower rate of 5' to 3' exonuclease activity of taq DNA polymerase compared to its polymerisation rate.

The principle advantage of probe based technologies is that multiple amplicons can be detected in the same tube. The number of amplicons that can be detected in real-time PCR is typically limited by the number of light channels found in the real-time PCR instrument. PCR Biosystems probe mixes are designed not to lose efficiency during multiplexing. PCR Biosystems real-time PCR probe mixes have been designed for use on a wide range of probe technologies including Taqman®, Molecular Beacons® and Scorpion probes®.

All PCR Biosystems real-time PCR mixes can be run under fast or standard cycling conditions.

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Product category: Enzymes

PCRBIO Rapid Extract PCR Kit

Rapid, convenient, single-tube DNA extraction
Produces high yield, PCR ready DNA in 15 minutes
Maximised PCR speed, yield and specificity
Powered by PCRBIO HS Taq Mix Red for direct gel loading
Ideal for complex templates
PCRBIO Rapid Extract PCR Kit combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy to use format. Eliminate the need for laborious and time-consuming DNA extraction methods with this simple, integrated extraction and amplification PCR kit powered by the latest advances in hot-start polymerase technology.

PCRBIO Rapid Extract PCR Kit has been developed for fast, efficient amplification of DNA from a variety of tissues and is particularly suited to solid tissue such as mouse tail or mouse ear. Sample processing is simplified and contamination risks minimised as DNA extraction is performed in a single tube, removing the need for multiple washing steps. Extracted DNA is amplified in a proprietary buffer system using PCRBIO HS Taq Mix Red. Our antibody hot-start polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and sensitivity. Primer-dimer formation and non-specific amplification are prevented giving superior specificity ideal for complex templates such as mammalian genomic DNA. PCRBIO HS Taq Mix Red contains a red tracking dye for direct loading on agarose gels.

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About us

Company details

PCR Biosystems offers a range of best-in-class kits and reagents for PCR and related technology. By combining our enhanced polymerases with highly-developed reaction buffers and novel hot-start chemistry PCR Biosystems is leading the development of PCR. Our PCR reagents have been developed to maximise yield and sensitivity in the simplest and most challenging of reactions.

Our portfolio covers a broad range of applications including, real-time PCR, endpoint PCR, high fidelity PCR, hot-start PCR, long PCR, PCR direct from crude samples and molecular diagnostic PCR.

All PCR Biosystems products have undergone comprehensive competitor product comparisons. On average we out perform all competitors in yield, specificity, sensitivity and speed.

PCR Biosystems produces, manufactures and distributes under a comprehensive quality management system, following ISO 9001:2008 standards.

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