Manufacturers

engine GmbH

Neuendorfstr. 17 , 16761 Hennigsdorf
Germany

Telephone +49 3302 5519983
Fax +49 3302 5519980
info@engine-gmbh.de

This company is co-exhibitor of
DiagnostikNet-BB Netzwerk Diagnostik Berlin-Brandenburg e.V.

Trade fair hall

  • Hall 3 / G52
 Interactive Plan

Hall map

MEDICA 2016 hall map (Hall 3): stand G52

Fairground map

MEDICA 2016 fairground map: Hall 3

Our range of products

Product categories

  • 03  Diagnostics
  • 03.02  Immunochemistry (Immunology)
  • 03.02.07  Immuno assay systems

Immuno assay systems

Our products

Product category: Immuno assay systems

engine Protein Arrays


engine offers different types of Protein Macroarrays. The corresponding cDNA expression libraries were validated and advanced based on more than fifteen years of experience in the field.

hEX1 engine Protein Macroarray
The hEX1 protein expression library was constructed by preparing cDNA from human fetal brain poly(A)+ RNA, using oligo (dT)-priming. The resulting fragments (larger than 500 bp) were cloned into a modified expression vector (pQE30NST) allowing protein expression to be induced by addition of IPTG to the growth medium. The resulting expressed proteins include an N-terminal RGS-HIS-Tag, which can be used for detection and purification purposes. 193,536 clones were picked, spotted and induced on PVDF-membranes and thereafter assayed for protein expression using an anti-HIS-antibody. Based on this screen, 37,830 putative expression clones were selected and re-arrayed into 384 well microtiter plates which constitute the hEX1-library. Spotted on high-density protein filters on two PVDF-membranes, the library is available as hEX1 part 1 and part 2 (Büssow et al., NAR1998). Most clones of this library have been 5’tag sequenced and annotated. The clone collection contains full-length as well as shorter cDNA clones representing full-length along with partial proteins. Some clones express artificial sequences derived from cDNA-construct as well as 5’ and 3’ UTRs.

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About us

Company details

engine – the biomarker company
We are a biotechnology company, located with headquarters in Hennigsdorf just outside Berlin and a production site in Berlin-Buch. Directions can be found here.

We provide our customers with a range of products in the field of protein array technology, enabling them to perform high throughput and multiplexed assays, by addressing up to 2 × 27,648 in situ expressed proteins in a single experiment.

We offer numerous engine Protein Macroarrays presenting human protein collections derived from different tissues. In addition, we offer customized protein arrays according to the individual needs of our customers. Sizes of the customized arrays range from ∼500 up to 13,000 individual clones, printed in duplicates.

Using our engine Protein Macroarrays, protein interactions with many types of molecules can be investigated. Our arrays can be applied to determine antibody profiles of complex sera, cross-reactivity of monoclonal antibodies, protein-protein interaction pairs, DNA/RNA binding proteins, etc.

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