Service

Fraunhofer IZI-BB e.V.

Am Mühlenberg 13 , 14476 Potsdam
Germany

Telephone +49 331 58187-0
Fax +49 331 58187-199
ivd-platform@izi-bb.fraunhofer.de

This company is co-exhibitor of
DiagnostikNet-BB Netzwerk Diagnostik Berlin-Brandenburg e.V.

Trade fair hall

  • Hall 3 / G52
 Interactive Plan

Hall map

MEDICA 2016 hall map (Hall 3): stand G52

Fairground map

MEDICA 2016 fairground map: Hall 3

Our range of products

Product categories

  • 03  Diagnostics
  • 03.01  Clinical chemistry
  • 03.01.02  DNA diagnosis kits
  • 03.01.02.01  Lab-on-a-chip

Lab-on-a-chip

  • 03  Diagnostics
  • 03.01  Clinical chemistry
  • 03.01.07  Gene tests / molecular biological diagnostic

Gene tests / molecular biological diagnostic

  • 03  Diagnostics
  • 03.01  Clinical chemistry
  • 03.01.07  Gene tests / molecular biological diagnostic
  • 03.01.07.02  DNA analysis

DNA analysis

  • 03  Diagnostics
  • 03.01  Clinical chemistry
  • 03.01.14  Rapid tests - clinical chemistry
  • 03.01.14.01  Point of care - testing systems

Point of care - testing systems

  • 03  Diagnostics
  • 03.02  Immunochemistry (Immunology)
  • 03.02.07  Immuno assay systems
  • 03.02.07.06  Enzyme immunoassay (EIA, ELISA, EMIT)

Enzyme immunoassay (EIA, ELISA, EMIT)

  • 03  Diagnostics
  • 03.02  Immunochemistry (Immunology)
  • 03.02.07  Immuno assay systems
  • 03.02.07.10  Heterogenous enzyme immunoassay

Heterogenous enzyme immunoassay

  • 03  Diagnostics
  • 03.02  Immunochemistry (Immunology)
  • 03.02.07  Immuno assay systems
  • 03.02.07.11  Homogenous enzyme immunoassay

Homogenous enzyme immunoassay

  • 03  Diagnostics
  • 03.02  Immunochemistry (Immunology)
  • 03.02.15  Rapid tests - Immunochemistry
  • 03.02.15.02  Infectious disease tests

Infectious disease tests

  • 03  Diagnostics
  • 03.05  Infectious Immunology
  • 03.05.05  Equipment and systems for microbiological diagnosis/virology
  • 03.05.05.01  Diagnostic agents / kits for microbiological diagnosis

Diagnostic agents / kits for microbiological diagnosis

  • 03  Diagnostics
  • 03.05  Infectious Immunology
  • 03.05.05  Equipment and systems for microbiological diagnosis/virology
  • 03.05.05.02  Equipment for microbiological diagnosis

Equipment for microbiological diagnosis

Our products

Product category: Diagnostic agents / kits for microbiological diagnosis

Assay Development

Experience and Expertise

The development of a microarray-based assay from a biomarker to a reliable and useful diagnostic tool needs long-time experience since many crucial steps have to be considered: The right type of interaction, the choice of surface (chemistry) and its quality control.

Types of Recognition Site

If a biomarker is detected, the type of recognition has to be chosen with appropriate care as it heavily affects the sensitivity and selectivity of the assay. Mostly, antibodies are used for the recognition of different non-nucleic acid based analytes. For nucleic acids the complementary strand is chosen and a hybridization is performed. Beside that, also different other recognition elements such as peptides, aptamers or artificial receptors may be used to enhance the performance of the assay.

Surface Chemistry

With regard to the type of receptor unit, the surface of the array slide has to match the chemical nature of the receptor and the analyte. On the one hand the receptor has to be immobilized onto the substrate using the appropriate coupling chemistry. On the other hand the analyte should not bind to the surface because unspecific binding leads to lower sensitivity and therefore an inferior assay performance. Thus, the right choice of surface modification combined with reducing the amount of unspecific binding is a fine balancing act which has to be taken into account.

Signal generation

The next step of development is the choice of labelling, which is needed to match the type of detection. For the Fraunhofer ivD-platform, so far, either a fluorescence or electrochemical read-out was demonstrated. With the kind of label the sensitivity can be tuned. In cases in which the sensitivity is not sufficient also on-chip amplification methods can be used to enhance the signal for the analysis.

Quality control

The last step on the way to a functional microarray is the quality control. Within a single array, different control spots have to be included to show that the assay has worked properly and no cross-contamination has occurred. But secondly, also the manufacturing of thousands of microarrays has to match quality control standards to fulfill different standardization protocols for the accreditation of ivD-products.

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