Reagents for PCR
Companies Biosan and Biolabmix are manufacturers of variety reagents for PCR (enzymes, standard and modified NTP and dNTPs, DNA Ladders, MasterMixes) and Immunobiochemicals (monoclonal antibodies, affinity purified antibodies, conjugates, immunosorbents) including unique antibodies to proteins of tick borne encephalitis virus (TBEV).
Hot Start Taq DNA Polymerase is the optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22 °C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70 °C. The PCR products obtained with Hot Start Taq DNA Polymerase are free from unspecific products and from primer-dimers.
reliable and reproducable quantification in qPCR
perfect for real time PCR
especially for diagnostic purposes
reaction set-up at room temperature
activation of enzyme during first heating
no change or optimization of protocol necessary
high specifity, reduced primer mismatch or dimers
Hot start PCR
Real time PCR
Amplification of complex genomic and cDNA templates
High specifity PCR
Taq DNA polymerase, highly purified from bacterial DNA. Concentrated up to 50 000 U/ml – by request.
Purified from E.coli strain carrying plasmid with the cloned gene encoding Thermus aquaticus DNA polymerase. Taq DNA polymerase catalyses 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, but possesses low 5’→3’ exonuclease activity.
Unit Definition: One unit of enzyme catalyses incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble polynucleotide fraction in 30 min at 70°C.
Activity assay: 50 mM Tris-HCl (pH 8.0 at 25°C), 50 mM NaCl, 10 mM MgCl2, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 50 μM [3H] dTTP, 0,25 mg/ml activated calf thymus DNA.
Storage conditions: -20° C in 50 mM Tris-HCl (pH 8.0 at 25°), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol and 1% triton X-100.
Quality control: Endo-, exodeoxyribonucleases, ribonucleases free.
Amplifications of DNA fragments by polymerase chain reaction (PCR).
DNA labelling with radionucleotides, digoxygenin or biotin.
T7 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
• Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Synthesis of unlabeled and labeled RNA that can be used:
• For hybridization, in vitro RNA translation
• As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
• In studies of RNA secondary structure and RNA-protein interactions, RNA splicing
Consensus promoter sequence:
M-MLV Reverse Transcriptase (RNase H Minus)
Description: Purified from E. coli strain carrying a plasmid with the cloned portion of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase. The enzyme possesses RNA- and DNA-dependent DNA polymerase activity, but lacks ribonuclease H activity.
Unit definition: One unit is the amount of the enzyme incorporating 1 nmol of dTTP into acid-insoluble form in 10 min at 37ºС in 50 mM Tris-HCl, pH 8.3, 40 mM KCl, 1 mM DTT, 6 mM MgCl2, poly A 2 A260/ml, 2 mM d(pT)12, 50 mM [H3]dTTP, 100 mg/ml BSA.
Storage conditions: 50 mM Tris-HCl, pH 8.0 (at 25 ºС), 100 mM NaCl, 1 mМ EDTA, 5 mМ DTT, 50 % glycerol, 0,1 % NP-40.
Store at –20 °C.
Quality control: Tested for the absence of exo-, endodeoxyribonuclease and ribonuclease activity.
v Production of templates for PCR
v First strand cDNA synthesis
v Construction of cDNA libraries
v Labeling and filling-in 3’-end-termini
v mRNA 5’-end mapping by primer extension analysis
v RNA and DNA sequencing
T4 DNA Ligase catalyzes the formation of a phosphodiester bonds between 5’ phosphate and 3’ hydroxyl termini in duplex DNA/RNA. This enzyme can join-blunt end and cohesive-end termini, repair single stranded nicks in duplexDNA, RNA or DNA/RNA hybrids.Purified from E. coli strain harbouring the plasmid that directs the synthesis of T4 DNA ligase.
Cloning of restriction fragments, joining linkers and adapters to blunt-ended DNA, gene (gene fragments) synthesis.
For most cohesive-end ligations, a 30 minute incubation at 20°C is sufficient. Incubations at 16°C for 4-16 hours are routinely used for the majority of applications.
Ligation of blunt-ends and single-base pair overhang fragments requires more enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Ligation can be enhanced by addition of PEG or by reducing the rATP concentration.
Each lot of T4 DNA ligase is tested for endonucleases/exonucleases, in a blue/white cloning assay.
T4 Polynucleotide Kinase catalyses transfer and exchange of Pi from g position of ATP to 5’-hydroxyl terminus of polynucleotides (double and single-stranded DNA and RNA) and nucleoside 3’-monophosphates. Polynucleotide kinase also catalyses the removal of 3’-phosphoryl groups from 3’-phosphoryl polynucleotides and deoxynucleoside 3’-diphosphates.
Purified from E.coli strain carrying T4 polynucleotide kinase overproducing plasmid.
Quality control: Endo-, exodeoxyribonucleases, ribonucleases, phosphatase free.
DNA or RNA 5'-end labelling.
Incorporation of phosphates in 5'-hydroxyle of oligonucleotides to allow subsequent ligation.
Removal of 3’-phosphoryl group.
E. coli uracil-DNA glycosylase catalyzes the release of free uracil from uracilcontaining DNA.
Uracil-DNA Glycosylase efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not
from oligomers (6 or less bases).
Our DNA Ladders are ideal for determining the size of double-stranded DNA from 50 to 10,000 base pairs.
Our highly purified dNTPs allow for synthesis of DNA PCR-products up to 20 kb.
We offer a variety of PCR MasterMixes from BioLabMix Ltd (http://www.biolabmix.ru/en/):
- MasterMixes for Reverse Trascription,
- Standard PCR,
- Real-Time PCR,
- Hot Start PCR,
- Long Range PCR
M-MuLV–RH First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit includes M-MuLV Reverse Transcriptase lacking RNase H activity. Universal kit for synthesis of first strand cDNA from mRNA or total RNA templates up to 10 kb.
Master-mix for Real-Time PCR
Ready-to-use solutions optimized for quantitative real-time PCR assay with sequence-specific probes and intercalating dye SYBR Green I. The master mixes include highly processive recombinant Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers should be added.
• Specificity – the optimized buffer reduces non-specific amplification and formation of primer dimers risks
• Sensitivity – detects low copy number targets
• Wide linear range – accurate quantification beginning from 10 – 100 copy of target molecules.
• Reproducibility and convenience – ready-to-use 2× master mix
Master-mix for Real-Time RT-PCR and RT-qPCR
Ready-to-use master mixes for one-step RT-PCR from different RNA templates. They include all the components required for a rapid, sensitive and reproducible RT-PCR (except for RNA template and primers)
• RT and PCR steps are performed in one tube – convenient in use plus reduced contamination risk
• The enzymes are highly sensitive in a broad dynamic range – accurate detection of RNA input (from 1 pg to 1 μg)
• Wide working temperature range (37 to 50 °C) for success with GC-rich and other difficult templates
• RT-PCR products can be loaded directly onto an electrophoresis gel (additional dyes available with BioMaster RT-PCR-Color)
• An inert blue dye eases the pipetting and application of reaction mixtures (BioMaster RT-qPCR SYBR Green
Master-mix for Real-Time Standard PCR
Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of 5 kb and shorter template
• Thermostable – half-life is more than 40 min at 95 °C
• Generates PCR products with 3’-dA overhangs
• Incorporates modified nucleotides
• Ready-to-use master mixes (2×) are convenient in use and reduce contamination risk
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+7 (383) 363 51 91 / +7 (383) 363 51 91