Simplified method for calibrating optical tweezers

29.07.2021

A team of researchers led by Prof. Cornelia Denz from University of Münster has developed a simplified method to perform the necessary calibration of optical tweezers. Shortening the measurement time helps to reduce the risk of damage to biological samples due to light-induced heating.

Measurements of biomechanical properties inside living cells require minimally invasive methods. Optical tweezers are particularly attractive as a tool. It uses the momentum of light to trap and manipulate micro- or nanoscale particles. A team of researchers led by Prof. Dr. Cornelia Denz from the University of Münster has now developed a simplified method to perform the necessary calibration of the optical tweezers in the system under investigation. Scientists from the University of Pavia in Italy were also involved.

Image: A black device with an illustration laid over it; Copyright: Pascal Runde

A microparticle held with optical tweezers in the microscope. Inset: Illustration of the held particle (magnified); shown in red is the light of the infrared laser used.

The results of the study have been published in Scientific Reports.

The calibration ensures that measurements of different samples and with different devices are comparable. One of the most promising techniques for calibrating optical tweezers in a viscoelastic medium is the so-called active-passive calibration. This involves determining the deformability of the sample under investigation and the force of the optical tweezers. The research team has now further improved this method so that the measurement time is reduced to just a few seconds. The optimized method thus offers the possibility of characterizing dynamic processes of living cells. These cannot be studied with longer measurements because the cells reorganize themselves during the measurement and change their properties. In addition, the shortening of the measurement time also helps to reduce the risk of damage to the biological samples due to light-induced heating.

In simplified terms, the underlying procedure to perform the calibration works as follows: The micro- or nanometer-sized particles are embedded in a viscoelastic sample held on the stage of a microscope. Rapid and precise nanometer-scale displacements of the specimen stage cause the optically trapped particle to oscillate. By measuring the refracted laser light, changes in the sample's position can be recorded, and in this way, conclusions can be drawn about its properties, such as stiffness. This is usually done sequentially at different oscillation frequencies. The team led by Cornelia Denz and Randhir Kumar, a doctoral student in the Münster research group, now performed the measurement at several frequencies simultaneously for a wide frequency range. This multi-frequency method leads to a shortened measurement time. The scientists used solutions of methyl cellulose in water at different concentrations as samples. These have a similar viscoelasticity to living cells.

COMPAMED-tradefair.com; Source: Westfälische Wilhelms-Universität Münster

More about the the WWU at: www.uni-muenster.de

Further exciting articles from the COMPAMED-tradefair.com editorial team:

Interview: Precise positioning: microscope stages with cobalt-samarium coated metal bands