SPECIFIC ANTIBODY CONCN: 1mg/ml by Lowry protein assay using BSA standard. Measured after dispensing and prior to freeze-drying. PRODUCT PRESENTATION : Lyophilised in PBS (0.01M, pH 7.4) without preservative.
ANTIBODY PREPARATION : This antibody product is produced by first immunising sheep with a single synthetic peptide which has the amino acid sequence : APTKAKRRVVQREKR This amino acid sequence corresponds to amino acid numbers 497-511 in the envelope gene gp120 protein of the BH-10 strain of HIV-1 (see Ratner, L. et al., Nature, 313, 277-284 (1985). The antibodies are isolated from the sheep hyperimmune serum by affinity chromatography using the above specified synthetic peptide coupled to Sepharose.
SPECIFICITY : The serological activity of the antibodies is checked by ELISA. PACKAGING : 1 x 2mg (2ml) vial
RECONSTITUTION : Reconstitute with 2ml of double distilled water.
Note : On reconstitution, a certain proportion of the antibody does not go back into solution due to partial aggregation during the freeze-drying process. This insoluble product can be removed by centrifuging at approximately 3000rpm for 5 minutes and then using the resultant supernatant. The proportion of insoluble aggregate will vary from lot to lot and possibly even from vial to vial.
STORE : Lyophilised product at +4oC. Reconstituted product should be stored in aliquots at -20oC.
PRODUCT CODE : BM 6064 SHELF LIFE : 10 years from date of manufacture SOURCE : Adenovirus Type 2 (Strain Adenoid 6) CULTURE SYSTEM : Hep-2 cell culture PRESENTATION : Harvested infected cells are treated to remove cellular components and ultracentrifuged to remove whole virus particles. Pure hexon antigen is obtained from the resulting supernatant by ion exchange chromatography and the antigen presented in 30mM BIS Tris buffer, pH 6.8 INACTIVATION : Whole infective virus particles removed by ultracentrifugation. Confirmation by attempted growth under original culture conditions. PROTEIN CONCENTRATION : mg/ml by absorption at 280nm. PRESERVATIVE : None STORAGE : Frozen (-700C). Sonicate before use. Avoid repeated freezing and thawing.
PRODUCT CODE : AJ 3152 SOURCE : Normal human serum/plasma.
PRESENTATION : Dispensed on the basis of dry weight. Supplied lyophilised from 0.2M NH4HCO3. The product may contain traces of buffer salts. It is recommended that the product be reconstituted using a phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.
SPECIFIC PURITY : Nominally greater than 96% ANALYSIS : SDS-PAGE and cellulose acetate electrophoresis show one band only. Albumin identity and content determined by Radial Immunodiffusion (RID) Total protein determined by the method of Lowry and by optical density using E280nm = 0.58 1cm,0.1% Electrophoretic and chromatographic data are consistent with a molecular weight of approximately 66kD.
DISEASE TESTING STATUS : Starting material tested and found negative for HBsAg, anti-HIV-1/2 and anti-hepatitis C.
Product category: Units and systems for hormone determination, Control material for hormone determination
Mouse Monoclonal Antibody to Human CG (hCG) and β Subunit
PRODUCT CODE : BE 1111 CLONE NO. : 0804048 IMMUNOGEN : Human recombinant hCG and β subunit. PRESENTATION : Protein A purified imunoglobulin in 0.1M borate buffer saline, pH 8.0. PURITY : Nominally ≥99% CONCENTRATION : 1mg/ml measured by optical density using E 280nm = 1.45 1cm, 0.1% SPECIFICITY : Recognises natural and recombinant hCG and β subunit. APPLICATION : Label antibody for the detection of hCG and its free β- subunit in RIA or ELISA. It is recommended that this clone be used with clone no. 2603048 as capture antibody. PRESERVATIVE : None. STORAGE : Below -20ºC
Founded in 1978, Aalto Bio Reagents is a leading provider of raw materials to the in-vitro diagnostics industry and to research laboratories globally. We serve the largest multinational companies in our industry with a broad range of purified human proteins; monoclonal and polyclonal antibodies; fungal, parasitic, bacterial and viral antigens; and disease state plasma for in-vitro diagnostic application.