SYnAbs S.A.

High affinity and extreme specificity monoclonal antibodies from rat species to target hormones, polysaccharides, post-translational modifications on DNA, RNA mutations, peptides, small molecules, poor immunogenic compounds, carbohydrates, lipids, small molecules, chemical compounds...

Applications such diverse as food testing (aflatoxin,...), drug abuse, auto-immune diseases (diabetes, arthritis...), infectious diseases (virus, antibiotic-resistant bacteria,...), cancer targets, hormone measurement, exosomes... 

High affinity and extreme specificity monoclonal antibodies from guinea pig species to target hormones, polysaccharides, post-translational modifications on DNA, RNA mutations, peptides, small molecules...

Applications such diverse as food testing (aflatoxin,...), drug abuse, auto-immune diseases (diabetes, arthritis...), infectious diseases (virus, antibiotic-resistant bacteria,...), cancer targets, hormone measurement, exosomes...

SYnAbs Mouse Immunoglobulin Easy Isotyping Kit or a.k.a. "MYstik™" provides a rapid and easy method (DOT technology) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations. This Plug&Play kit includes ready-to-use reagents necessary to analyze 24 samples in less than 1h30. Buffer solutions are color-coded to simplify pipetting steps.

The method is based upon robust EIA enzyme immunoassay technique:  SYnAbs unique rat anti-mouse monoclonals are highly specific to each of the common light and heavy chains of mouse species and can discriminate between IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, kappa chain and lambda chain. Samples are placed on the strip without any predilution. If desired isotype mAb is present, it will bind to rat mAb by affinity. Note that only specific mAb and none the irrelevant molecules will bind. That counts for the specificity of the test. The second mAb is then added, this one conjugated with the enzyme. A colorgenic enzyme substrate is added. The interaction between the substrate and the captured enzyme generates visible color or dot.

Immunoaffinity chromatography a.k.a. IAC is a type of chromatography in which the stationary phase is composed with an antibody. This technique represents a special sub category of affinity chromatography in which a biological agent is used for its strong and specific binding capacities to a target compound.

SYnAbs routinely used immunoaffinity columns for the purification of its mouse and rats off-the-shelf monoclonal antibodies and has decided to offer to its customers the possibility to bind SYnAbs mAbs or your custom mAbs to the resin of your choice. You can esaily purifiy mycotoxins antigens, but also rat or mouse antibodies,...

You'd like to purify mouse monoclonal antibodies? Why not choose and pick up one of our LOU products?
You'd prefer to purify rat monoclonal antibodies? You should definitely choose our MARK tools!

SynAbs is offering a vast catalog, including more than 1,000 monoclonal antibody references, with a specialization in secondary monoclonal antibodies targeting multiple repertoires:

• anti-mouse  secondary monoclonal antibodies,
• anti-rat secondary monoclonal antibodies,
• anti-human monoclonal antibodies
• anti-rabbit monoclonal antibodies 
• anti-species monoclonal antibodies: anti-pig, anti-horse, anti-chicken, anti-baboon, anti-bullock, anti-dog, and anti-rabbit mAbs

• Plasmid production
• Preparation of RNA
• Preparation of genomic DNA
• Vector construction
• Expression and purification of recombinant proteins
• Sequencing of monoclonal antibodies
• In vitro transcription
• PCR and real time PCR

Our molecular biology services are ideal if you wish to:
- To obtain quickly plasmid DNA in any quantity ?
- To make a customised vector ?
- To obtain a transcript or a protein where you don’t have the gene ?
- To purify a protein expressed fromyour own or a customised vector ?

During recent years, plasmid purification has become a major activity of RD-Biotech.
We have amplified or created over 600 plasmids per annum giving us unrivaled expertise in producing plasmids for applications in research or preclinical studies.
Whether you need 1 mg of DNA for an R & D project or 500 mg of high quality endotoxin free DNA for in vivo or a sensitive in vitro applications, we can fulfill your requirements.

RD-Biotech is able to provide 
- A 10 mg quantity in 3 days or 100 mg in 6 days!
- An endotoxin level guaranteed below 0.1 EU / µg DNA
- Large capacity to produce guaranteed quantities from milligrams to several hundreds od milligrams. 
- Uniform batches of 90% supercoiled DNA
- We also offer Pre-and Post-production controls
- Selection of the right clone to be amplified, after restriction pattern analysis, 
- Banking of your plasmid or clone for subsequent use
- Other services on request available are:

* Bacterial transformation using a strain of choice (Application and DNA dependant)
* DNA adjusted to a specific concentration
* Filtered DNA
* Aliquoting
* Determination of bacterial endotoxins (LAL / Limulus Amebocyte Lysate assay)
* Sterility tests


Applications:

1. Transfection of mammalian cells
2. in vivo experiments (injection into animals)
3. Preclinical programs
4. Toxicology Studies
5.  Applications in Research & Development

RD-Biotech has laboratories dedicated to the production of eukaryotic cells capable of providing small pilot batches to large-scale amplifications that meet the needs of its customers:

- Production of supernatant batches from a few milliliters to several hundred liters 
- Provision of a few million to several billion cells per batch

The production platform offers cell amplification of many cell types, in specialized units (dedicated laboratories, clean rooms under a controlled atmosphere...)

RD-Biotech provides the cells in the desired format, depending on the intended applications:

- Frozen Vials
- Culture plates (96 wells, 24 wells, 12 wells, 6 wells...)
- Boxes of culture flasks of any volume
- Alternative solutions available on request

RD-Biotech provides an appropriate safe express delivery system along accompanied by a production and quality control report which includes:

- A test for mycoplasma
- A test of cell viability
- Stability Control

RD-Biotech expert researchers have developed cell culture systems for most experimental models: 

• Amplification of cell lines

We have a strong experience in expanding adherent or suspension mammalian cell cultures using standard lines (eg CHO, HEK, HeLa, Cos etc) or lines provided by the customer
Amplification of cells according to a procedure provided by the customer or optimized by RD- Biotech.

• Production of CACO-2 cells

Expansion of these cells is performed under perfectly controlled and monitored conditions in specialised media.
Cells are supplied in 96-well plates on specific substrates, for applications in pharmacology.

• Production of primary cells (skin cells, adipocytes, keratinocytes etc...)

Adaptation of culture conditions with respect to passage number for each specific cell type.
Close culture monitoring and cell supplied in the desired format.

• Production of blood cells (from peripheral blood, cord blood, etc...)

Isolation of cells from blood samples: PBMCs (peripheral blood mononuclear cells), monocytes, lymphocytes, CBMC (Cord blood mononuclear cells)…
Cell sorting and preparation of specific cell subtypes (eg CD4+, CD34+, etc...)

• Production of cells for applications in virology

CELL BANK PREPARATION
This service includes the establishment of Working and Master cell banks stored in liquid nitrogen under controlled conditions!

- Mycoplasma test upon arrival of cells
- Monitored amplification of cells
- Preservation and storage in 1 to 5 ml cryovials (1 to 5 million cells each)
- Quality control after thawing

DEVELOPMENT OF CELL LINES: Stable cell lines
RD-Biotech offers a personalized service to define the best strategy for expression of recombinant proteins or recombinant monoclonal antibodies in a host cell of choice:

- A feasibility study to determine the best expression system and to define the optimal conditions for the expression of your recombinant protein.
- Choice of the host cell line: eg HEK293, CHO...
- Various transfection protocols available such as lipofection, electroporation, etc…
- Optimization and control of vectors: plus the in-house capability for vector construction 
- Adaptation to complex projects with a need for expression of different entities.

TRANSIENT TRANSFECTION PLATFORM
RD-Biotech has developed a platform to produce recombinant antibodies or proteins in a fortnight using transient transfection in microgram to milligram quantities.

ELISA, Western-blotting, Flow Cytometry etc.

RD-Biotech lends its expertise and immunoassay platform to the development of a series of Immunological detection methods: 

-We develop analytical methods according to the customer's specifications. 
-Analysis of samples by methods approved by the customer. 

For various applications:
-Animal and human in vitro diagnosis 
-Quality control 
-Research 
-Clinical Studies

-ELISA: Method development
RD-Biotech produces ELISA based protocols based on customer specification. 
RD-Biotech carries out an initial feasibility study to determine optimal parameters for the study such as: antibody choice, selection of raw materials, development and optimisation of each step (e.g coating micro-plates, staining, etc…) 

-TEST 1: Simple indirect sandwich test: using extracted or purified protein coated wells stained using a polyclonal rabbit antibody, or a commercial peroxidase coupled antibody appropriate to the species under test.
-TEST 2: Immuno-competition assay: wells coated with specific antibody, incubated and then stained with a mixture of peroxidase-labeled protein and unlabeled purified protein.
-TEST-3: Indirect or direct double sandwich test (depending on the availability of specific antibody): wells are coated with the antibody followed by incubation with either a second antibody or the purified protein. Staining can be either direct if the second antibody is conjugated or indirectly with a third species specific conjugated antibody.
Different parameters are measured for optimizing each strategy (eg. coating of micro-wells, sample incubation times and staining).
Data processing: All absorbance measurements are analyzed by BPL and saved as Excel files; Results are also recorded in a bench notebook and copies are available to the customer upon request. 

After this initial stage, RD-Biotech can identify the best methodology to use, specifying optimal operating conditions. The report provides a set of dose performance curves done under various conditions (eg.test sample volume, number and duration of incubation, enzyme conjugate) and exact details of all protocols performed.

The customer can then decide whether to initiate the transferable development phase of the robust ELISA technique we have developed.


- RD-Biotech: Analytical Services for ELISA, Western-blotting, flow cytometry, …
RD-Biotech samples analysis is performed using commercial kits or with a customer-validated protocol. A raw data report is then sent to the customer.

Types of analysis:

- Titration of immune responses by ELISA
- Immune response caracterization by ELISA or by a Western-blotting (multi-track system, multiscreen)
- Determination of cytokine profiles from different types of samples (plasma, culture supernatant, biological fluid, etc…) by ELISA 
- Cell sorting by flow cytometry

SYnAbs and RD Biotech have developed a one-stop-offer for custom bioproduction up to 300L scale for recombinant antigens, recombinant proteins and antibodies of any format (fragments, VHH, bispecific...) through E.Coli, wheat-germ, CHO, HEK, and hybridoma cell lines expression system including Up-Stream Processing (roller-bottle, CELLines, bioreactors...), Down-Stream Processing (protein A, G, IAC,...), aliquoting, freeze-drying activities amongst other activities.