GLUCOSE (5 MIN.)
Reagent kit for quantitative estimation of glucose in serum or plasma.
Product Highlights :
• Low blank .
• End Point formulation with five minutes incubation.
• Suitable pack size as per customer need.
• Linearity & Accuracy as per international standard.
BACKGROUND AND SYNOPSIS:
Conventional methods like Ferricyanide, Nelson-Somogyii, Folin-Wu, O-Toludine involved lengthy procedures and were cumbersome. Some of these methods involved steps like precipitation, filteration and boiling. These methods have drawbacks, are non-specific, imprecise, insensitive and inaccurate.ENZOPAK Glucose is based on GOD/POD method (Glucose oxidase /Peroxidase) as described by Trinder and is specific and accurate. The method selectively estimates β-D-Glucose in serum or plasma.
Glucose oxidase oxidises the specific substrate, β-D-glucose, to gluconic acid and hydrogen peroxide is generated. Hydrogen-peroxide thus produced is acted upon by peroxidase and oxygen is liberated. The liberated oxygen is transferred to chromogen system consisting of 4-aminoantipyrine and phenolic compound to produce red quinoneimine dye. The intensity of colour is directly proportional to the concentration of glucose and is measured photometrically at 505 nm(500-540 nm or with GREEN filter)