CaptSure DIY ELISA
Powered by Lightning-Link®
Immunoassays are the gold-standard method for the detection of proteins and biomarkers in discovery processes. However, ELISA development usually involves costly plate coating, antibody pair screening, long development and optimization times, and variable success rates. These limitations are now solved by CaptSure DIY ELISA.
CaptSure DIY ELISA is the latest cutting-edge ELISA kit with a built-in system for antibody labeling, enabling the rapid and easy development of in-house ELISAs using any antibody pair while eliminating the need to perform plate coating procedures.
Furthermore, the CaptSure DIY ELISA enables you to reduce the amount of capture antibody required, the ability to measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.
The CaptSure DIY ELISA optimized protocol results in faster ELISA development, reduced overall costs, and a greater assay flexibility to increase the research output with a reduced chance of error.
How does CaptSure DIY ELISA work?
CaptSure DIY ELISA is powered by the combination of Expedeons proprietary CaptSure and Lightning-Link technologies.
Unlike standard ELISA systems, the CaptSure DIY ELISA kit has a ready-to-go CaptSure assay plate, pre-coated with our CaptSure antibody, which is specific for the CaptSure peptide. The capture antibody is quickly and easily conjugated with the Lightning-Link CaptSure peptide supplied within the kit. Once added to the plate, the interaction between the CaptSure antibody and the CaptSure peptide immobilizes the immuno-complex to the plate.
The detection antibody is similarly conjugated with HRP using the Lightning-Link HRP conjugation reagent, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.
Lightning-Link is the worlds fastest conjugation technology to label antibodies and proteins by targeting free amine groups. As amine groups are ubiquitous and numerous in antibodies, any antibody can be labeled with the Lightning-Link CaptSure peptide and Lightning-Link HRP kits.
This labeling protocol is comprised of just a few simple steps:
Resuspend the lyophilized Lightning-Link CaptSure peptide and Lightning-Link HRP with the solution of capture and detection antibodies, respectively;
Incubate each at room temperature for the specified times;
Quench the reactions, and your labeled capture and detection antibodies are ready to use for the ELISA.