In 1945, Coombs, Mourant and Race described the use of anti-human globulin serum for detecting red cell-bound non-agglutinating antibodies. In 1957, Dacie et al showed that the antibodies present in antiglobulin sera were directed against certain components of complement. Anti-human globulin reagents detect non-agglutinating antibody molecules as well as molecules of complement attached to red cells following in vivo or in vitro antigen-antibody reactions.
When used by the recommended techniques, the reagents will react with immunoglobulins and/or complement attached to the red cell surface, resulting in agglutination (clumping) of adjacent sensitised cells. Cells not sensitised will not be agglutinated.
Lorne Polyspecific Anti-Human Globulin Elite Clear and Anti-Human Globulin Elite Green reagents contain anti-IgG derived from rabbits with non-specific activity removed by absorption and murine monoclonal IgM anti-C3d, Clone BRIC-8. The antibodies are diluted in a buffered solution containing bovine albumin. Each reagent is supplied at optimal dilution for use with spin tube techniques (DAT & IAT).
Levine and Stetson discovered the Rh blood group system in 1940. Apart from D the other major Rh antigens are C, E, c and e. The D antigen is highly immunogenic; the C and e antigens are less immunogenic than E and c. The corresponding antibodies are all clinically significant since they may cause both Transfusion Reactions and Haemolytic Disease of the Newborn.
The reagents will cause direct agglutination (clumping) of test red cells that carry the C and/or D and/or E antigen. No agglutination generally indicates the absence of the corresponding Rh antigen.
Lorne Monoclonal IgM Anti-Rh blood grouping reagents are low protein reagents containing human monoclonal antibodies diluted with sodium chloride, bovine albumin and macromolecular potentiators. Each reagent is supplied at optimal dilution for use with slide, tube, gel card and microplate techniques.
Lorne Laboratories is the only independent company in England that for over forty years has sought to provide a simple and total solution for Blood Transfusion professionals across the world. Lorne was created by biomedical scientists who believed that the manufacture and distribution of reagents and associated products for blood transfusion could be provided professionally, ethically and with quality at the centre of all that it did. Happy to be different
Today, the Lorne team believes that providing high quality and affordable solutions can make a difference to those blood transfusion professionals in over 100 countries in which it operates. This vision has been backed by years of investment to ensure that the finished product provides reassurance to all that use it and has earned Lorne a deserved reputation for quality. The Lorne management believes in providing the following benefits to all its customers:
A one-stop solution for blood transfusion professionals Quality products professionals can trust Competitive pricing Great customer service